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. 2013 May 21;4:1882. doi: 10.1038/ncomms2879

Figure 1. 14-3-3 protein controls proper SAC inactivation in a γ-subtype-dependent manner.

Figure 1

(a) Forty-eight hours after transfection with an indicated siRNA, HeLa cells were stained with anti-H3-pSer28 (red) and 4',6-diamidino-2-phenylindole (blue). Immunoblotting with anti-H3-pSer28 and anti-α-tubulin (loading control) are also shown in insets. (b) The effect of 14-3-3γ knockdown on cell-cycle progression after DTB synchronization. (c) Live-cell imaging analyses using H2B-GFP-expressing HeLa cells. Box-and-whisker plots show the elapsed time that cells spent in mitosis from nuclear envelope breakdown (t=0) to anaphase onset (n≥60 from two independent experiments). (d) The effect of 14-3-3γ silencing on KT-associated BubR1. Scale bar, 10 μm. (e, f) The effects of BubR1 or Mad2 silencing on mitotic arrest induced by 14-3-3γ depletion. Mitotic indices are shown as mean±s.e.m. from three independent experiments (n>200 each). *P<0.01; Student’s t-test.