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. 2013 May 21;4:1882. doi: 10.1038/ncomms2879

Figure 5. Plk1–Ser99 phosphorylation is dispensable for mitotic entry after release from a second block.

Figure 5

(a) The evaluation of Plk1 silencing using each target sequence of Plk1 (#1 or 3′-untranslated region (UTR)). (b) The effect of Plk1 silencing on mitotic entry after DTB synchronization. (c) The evaluation of mitotic index after the replacement. (d) The effect of Plk1 silencing on spindle bipolarity. Examples of monopolar or bipolar spindle formation are shown in the left. (e, f) The effect of the replacement with the indicated Plk1 (e) or 14-3-3γ silencing (f) on bipolar spindle formation. Data were obtained from two independent experiments (n>100; d–f). GAPDH, glyceraldehyde 3-phosphate dehydrogenase.