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. 2013 Apr 19;41(11):5679–5691. doi: 10.1093/nar/gkt277

Figure 1.

Figure 1.

RbpASc activates transcription from a range of σHrdB-dependent promoters in vitro. (A) Multi-round in vitro transcription reactions using the rrnDp3 promoter as template (22) and including core RNAP (75 nM) σHrdB at σHrdB: RNAP ratios of 0, 0.5, 1, 1.5 or 2, or 3, in the presence (grey bars) or absence (black bars) of excess RbpASc (40 μM). rrnDp3 transcription products are normalized relative to the highest signal. Note that these preparations of core RNAP have residual levels of σHrdB. (B) Multi-round in vitro transcription reactions contained core RNAP (75 nM) σHrdB (375 nM), RbpASc (750 nM) and DNA templates generated by PCR using primers listed in Supplementary Table S2. Data are presented as fold-difference relative to reactions lacking RbpA. Transcript levels were quantified by phosphorimaging from triplicate data, and standard deviation is indicated.