Figure 5.

Inducible expression of d2EGFP. Stable HEK293 Tet-On® -derived cells expressing the indicated reporter constructs were treated with a 1 µg/ml doxycycline to induce reporter gene expression. (A) Cells were collected at the indicated for flow cytometric analysis. Similar results were obtained in three independent experiments. (B) The mean increase in green florescence at each time point, determined from experiments in (A), is presented. (C) The results in (B) are also presented as a percentage of fluorescence detected by 24 h. Each value in (B) and (C) represents the mean (±SEM) from three independent experiments. The curves in panels (B) and (C) were significantly different by two-way ANOVA (P < 0.0001).