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. 2013 Apr 22;288(23):16235–16246. doi: 10.1074/jbc.M113.458695

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — A, the α-TM-D137L construct used to generate the TG mice. HGH, human growth hormone; B, TM region from a representative two-dimensional difference gel of myofibrillar proteins from 4-month-old NTG and TG mouse hearts. Myofibrillar fractions were labeled separately and equally mixed. Channel 1, Cy5-labeled α-TM (green); channel 2, Cy3-labeled α-TM-D137L (red); channel 3, merged image of channels 1 and 2. 81.3 ± 1.5% replacement (total α-TM-D137L/total α-TM) in TG mouse hearts was detected (n = 11). P-, phosphorylated protein.