TABLE 3.
Mass spectrometric analyses of the SecY(L127pBpa) cross-linking products
A non-UV irradiated (not cross-linked, −UV) and a UV-irradiated (cross-linked, +UV) sample of SecY(L127pBpa) purified from whole cells was separated on 5–15% SDS gels and the proteins were visualized by Coomassie staining. Gel areas around the bands that were detected by α-YidC antibodies (Fig. 3A) were cut out from the −UV and +UV lanes. Following in-gel trypsin digestion, the samples were analyzed by mass spectrometry. Shown is the quantification for YidC peptides.
| Proteina | Molecular massb | Gel molecular massc | Relative intensity (−UV/+UV)d | Coveragee | Peptidesf |
|---|---|---|---|---|---|
| kDa | |||||
| YidC | 61.5 | 55 | 0.60 | 46.7 | 17 |
| 65 | 0.01 | 50.2 | 22 | ||
| 92 | 0.00 | 34.1 | 11 | ||
| 130 | 0.00 | 27.6 | 12 | ||
a Protein identified.
b Calculated molecular mass.
c Molecular mass of gel slice determined by extrapolation.
d Relative intensity observed in gel slices from the control lane (−UV) compared to the +UV lane.
e Sequence coverage of total sequence by detected peptides.
f Number of peptides detected.