TABLE 1.
Name | Direction | Sequence |
---|---|---|
Mouse RT-QPCR primers | ||
Gapdh | Forward | 5′-GCTTGTCATCAACGGGAAGC-3′ |
Reverse | 5′-TTTGATGTTAGTGGGGTCTCGC-3′ | |
Ttf-1 | Forward | 5′-AAAACTGCGGGGATCTGAG-3′ |
Reverse | 5′-TGCTTTGGACTCATCGACAT-3′ | |
Srebf1 | Forward | 5′-CCAGAGGGTGAGCCTGACA-3′ |
Reverse | 5′-AGCCTCTGCAATTTCCAGATCT-3′ | |
Srebf2 | Forward | 5′-TGTGAACCTGGCCGAGTGT-3′ |
Reverse | 5′-CGCTGTCAGGTGGATCTCAA-3′ | |
Hmga2 | Forward | 5′-GGGCGCCGACATTCAAT-3′ |
Reverse | 5′-ACTGCAGTGTCTTCTCCCTTCAA-3′ | |
Human RT-QPCR primers | ||
GAPDH | Forward | 5′-GGAGTCAACGGATTTGGTCGTA-3′ |
Reverse | 5′-GGCAACAATATCCACTTTACCAGAGT-3′ | |
TTF1 | Forward | 5′-CGTTCTCAGTGTCTGACATCTTGA-3′ |
Reverse | 5′-CCTCCATGCCCACTTTCTTG-3′ | |
SREBF1 | Forward | 5′-GCTCCTCCATCAATGACAAAATC-3′ |
Reverse | 5′-TGCAGAAAGCGAATGTAGTCGA-3′ | |
SREBF2 | Forward | 5′-CGAATTGAAAGACCTGGTCATG-3′ |
Reverse | 5′-TCCTCAGAACGCCAGACTTGT-3′ | |
HMGA2 | Forward | 5′-CCCAAAGGCAGCAAAAACAA-3′ |
Reverse | 5′-GCCTCTTGGCCGTTTTTCTC-3′ | |
ChIP QPCR primers | ||
C9Orf5 (distal) | Forward | 5′-TGTCTGCCTTAAAAGCTTGTGTTC-3′ |
Reverse | 5′-TTTGCTCCAGTACTTTGCACACTT-3′ | |
C9Orf5 (proximal) | Forward | 5′-GGTATTCTAGTATGCAGCTTGGGTTT-3′ |
Reverse | 5′-GAAGCTCCTTTCACTCATTCGTTAA-3′ | |
SREBF2 (distal) | Forward | 5′-GAAGCGTGAATTGGCCTTATG-3′ |
Reverse | 5′-TCTGACCCTTTCCCGTTGAT-3′ | |
SREBF2 (proximal) | Forward | 5′-GCAAAGCAGAAGACGTAAAATCC-3′ |
Reverse | 5′-CACGGAGGCTTGACAAGGTT-3′ | |
SPB (distal) | Forward | 5′-CCGAAGGCTGTAGGGAAGAA-3′ |
Reverse | 5′-GCTCAGGAACACTAGGGATTGC-3′ | |
SPB (proximal) | Forward | 5′-TCCACCCACAAAAGCACTGTAG-3′ |
Reverse | 5′-TCTCCCTAGTGGAGGCTCACA-3′ | |
HMGA2 3′-UTR psiCHECK2 site-directed mutagenesis primersa | ||
Δ1 | Forward | 5′-CAGGGGACACAGCTTAAGGTACCCTTTTAATTACTG-3′ |
Reverse | 5′-CAGTAATTAAAAGGGTACCTTAAGCTGTGTCCCCTG-3′ | |
Δ2 | Forward | 5′-GCTGCTATACACAAGGGTACCGAAAAAAACTTACTG-3′ |
Reverse | 5′-CAGTAAGTTTTTTTCGGTACCCTTGTGTATAGCAGC-3′ | |
Δ3 | Forward | 5′-GCCCTGCTTTTGCATGGTACCTCAAAAATATGTG-3′ |
Reverse | 5′-CACATATTTTTGAGGTACCATGCAAAAGCAGGGC-3′ |
a The KpnI cut site is underlined.