FIGURE 2.

Binding of multiple POT1-TPP1 proteins to wild-type and mutant telomere DNA templates. EMSA experiments of hT48wt (A), hT48GG→CC (B), and hT48→40ΔGG (C) constructs, respectively. EMSAs were performed with DNA concentration remaining constant in each lane and POT1-TPP1 concentration increases from 0 – 2× molar excess/POT1 binding site on the DNA template (in 0.2× molar excess increments from left to right). Numbers in A indicate the number of POT1-TPP1 heterodimers bound to the DNA. D, quantification of the EMSA data for POT1-TPP1 binding to the three oligonucleotides described.