FIGURE 10.

Selective depletion of dimeric cystatin C in blood plasma. Quantification of cystatin C in the fractions of gel filtration of plasma samples on a column of Superdex 75 GL 10/300 (A and B). In the control sample (black line) stabilized dimeric cystatin C was added to normal human plasma, resulting in a concentration of 1.7 mg/liter (endogenous monomeric cystatin C = 0.9 mg/liter). The oligomer-specific antibodies were added to the plasma sample 2 h before fractionation (red and blue lines). B, plot displaying the percentage of dimeric cystatin C of the total cystatin C (monomeric + dimeric) after the addition of increasing amounts of oligomer-specific antibodies.