FIGURE 4.

Mitotic phosphorylation results in the dissociation of HDAC1 and -2. Total cell lysates (500 μg) from cycling and nocodazole (Noc)-treated HeLa cells were incubated with 3.0 μg of rabbit polyclonal anti-HDAC2 (A) or anti-HDAC1 (B) (poly) antibodies. The co-immunoprecipitations were checked by immunoblotting with mouse monoclonal anti-HDAC1 and -2 (mono), or rabbit polyclonal anti-HDAC1 and -2 (poly) antibodies. Immunoprecipitated and immunodepleted fractions are indicated as IP and ID, respectively. The phosphorylated form of HDAC2, which has reduced mobility in SDS-PAGE, is shown as HDAC2*.