Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Apr 23;288(23):16518–16528. doi: 10.1074/jbc.M112.440446

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 7. — CK2 mediated increased phosphorylation of HDAC2 during mitosis. A, whole cell lysates from TBB-nocodazole (Noc) or quinalizarin-nocodazole-treated HeLa cells were analyzed by SDS-PAGE (left) and two-dimensional gel electrophoresis (right) and subsequently immunoblotted (IB) with anti-HDAC2 antibody to check for phosphorylation levels. β-Actin levels in extracts were also examined on an immunoblot to demonstrate that there was equal loading of protein from each of the lysates. B, immunoblot analysis of V5 and HDAC2 from cellular extracts of Flp-In 293 cells stably expressing HDAC2-WT-V5 (WT) or HDAC2-M3A-V5 proteins (M3A), treated with nocodazole (Noc) or okadaic acid (OA). HDAC2*, phosphorylated form of HDAC2.