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. 2013 Apr 23;288(23):16518–16528. doi: 10.1074/jbc.M112.440446

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Inhibition of protein kinase CK2 activity prevents dissociation of HDAC1 and HDAC2 during mitosis. A and B, total cell lysates (500 μg) from nocodazole (Noc)- and TBB-nocodazole-treated (A) or from nocodazole- and quinalizarin-nocodazole-treated (B) HeLa cells were immunoprecipitated with 3.0 μg rabbit polyclonal anti-HDAC1 or anti-HDAC2 or control isotype-specific non-related rabbit IgG antibodies. The co-immunoprecipitates were checked by immunoblotting with mouse monoclonal anti-HDAC2 and rabbit polyclonal anti-HDAC1 antibodies (left panels of A and B) or mouse monoclonal anti-HDAC1 and rabbit polyclonal anti-HDAC2 antibodies (right panels of A and B). HDAC2*, phosphorylated form of HDAC2. IP and ID, immunoprecipitated and immunodepleted fractions, respectively.