TABLE 1.

Phosphoacceptors identified after purification of M. tuberculosis GlgE from the E. coli strain co-expressing M. tuberculosis PknB

Sequences of the phosphorylated peptides identified in GlgE as determined by mass spectrometry following tryptic digestion are indicated, and phosphorylated residues (pT or pS) are shown in bold.

Phosphorylated tryptic peptide sequence of GlgE purified from pCDFDuet co-expressing PknB	Number of detected phosphate groups (LC/MS/MS)	Phosphorylated residue(s)
[5–17] AIGTEpTEWWVPGR	1	Thr-10
[51–63] EGHEAVAApTLVVR	1	Thr-59
[80–90] VLPTPpSEPQQR	1	Ser-85
[141–163] LDAGQGEpTELSNDLLVGAVLLER	1	Thr-148
[185–192] TPGDPVpTR	1	Thr-191
[185–211] TPGDPVTRpTALALTPEIEELLADYPLR	1	Thr-193
[361–379] QWFTELPDGpTIAYAENPPK	1	Thr-370