TABLE 1.
Phenotypes observed in Hmgntm1/tm1 mice
The abbreviations used are as follows: IpGTT, intraperitoneal glucose tolerance test; IC, inspiratory capacity; ERV, expiratory reserve volume; VC, vital capacity; TLC, total lung capacity; FVC, forced vital capacity.
| Screens | Test | Phenotypea |
||
|---|---|---|---|---|
| Hmgn1tm1/tm1 | Hmgn3tm1/tm1 | Hmgn5tm1/tm1 | ||
| Behavior | Open field | Decreased rearing activity | No | No |
| Acoustic startle and prepulse inhibition | Lower acoustic startle activity | Decreased PPI | Decreased PPI | |
| Neurology | Modified SHIRPA protocol | No | No | No |
| Grip strength | No | Reduced | No | |
| Nociception | Hot plate assay | No | Hypoalgesia | No |
| Clinical chemistry and hematology | Clinical chemistry | No | See website | See website |
| Hematology | No | No | Changes in the red blood cell count | |
| Simplified IpGTT | No | Increased fasting-induced body mass loss, increased peak glucose | Sex-specific effects on basal glucose level and glucose tolerance | |
| Steroid metabolism | Corticosterone level | Decreased in females | Decreased in females | No |
| Testosterone level | No | Increased in males | No | |
| Immunology | FACS, leukocyte proportions | No | No | Higher frequency of CD8+ T cells and lower frequency of granulocytes |
| Bioplex: immunoglobulin levels | No | No | Higher IgA | |
| Allergy | IgE level | No | No | Low IgE |
| Lung function | Pulmonary function test | No | No | Sign differences in lung volumes (IC, ERV, VC, TLC, and FVC), flow rates (FEV100), mechanical properties (Cchord); emphysema-like phenotype |
| Molecular phenotyping | Genome-wide transcriptome analysis | Differential gene expression in liver, spleen, thymus and brain | ||
a See the Mouse Clinic website for additional data.