FIGURE 6.

Cytokines modulate NOTCH signaling activity through NF-κB and MAPK pathways in rat NP cells. The activity of NOTCH responsive reporters 12xCSL (A and B) and 4xCBF1 (C and D) following IL-1β and TNF-α treatment for 24 h with or without inhibitors is shown: SM7368 (SM), SP60025 (SP), SB203580 (SB), or PD98059 (PD). Increased activation of both the reporters by IL-1β (A and C) or TNF-α (B and D) was suppressed by NF-κB and MAPK inhibitors. Values shown are mean ± S.E. of three independent experiments; *, p < 0.05.