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. 2013 Apr 15;288(23):16761–16774. doi: 10.1074/jbc.M112.446633

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — MAPK regulation of cytokine-mediated induction of NOTCH2 promoter activity in rat NP cells. NP cells were cotransfected with NOTCH2 promoter construct along with either DN-p38α (A) or DN-p38β2 (B) or DN-p38δ (C) or DN-p38γ (D) or DN-ERK1 (E) or DN-ERK2 (F) or empty backbone vector pcDNA3. Transfected cells were treated with IL-1β or TNF-α, and reporter activity was measured 24 h after the treatment. Co-transfection with DN-p38α, DN-p38β2, DN-p38δ, DN-ERK1, and DN-ERK2 resulted in nearly complete suppression of cytokine-dependent induction of the NOTCH2 promoter activity. However, cotransfection of DN-p38γ did not block IL-1β-mediated induction of the reporter activity, and there was a small inhibition in TNF-α treatment group. Values shown are mean ± S.E. of three independent experiments; *, p < 0.05.