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. 2013 Apr 16;288(23):16775–16787. doi: 10.1074/jbc.M113.467159

FIGURE 1.

FIGURE 1.

TMPMs bind and block efflux of NR. A, binding of FITC-tagged TMPMs with cell surface of Cdr1p-overexpressing S. cerevisiae cells. Confocal images of interacting TMPMs with control (AD1-8u−) and AD-CDR1-overexpressing cells are shown. B, confocal images of noninteracting TMPMs with control (AD1-8u−) and AD-CDR1-overexpressing cells. C, binding of interacting (1, 2, 4, 8, 10, and 11) and noninteracting (3, 5, 6, 7, 9, and 12) FITC-tagged TMPMs with cell surface of AR (Gu5) cells. D, intracellular accumulation of NR in AR (Gu5) cells in the absence of TMPMs, which was treated as control. E, intracellular accumulation of NR in AR (Gu5) cells in the presence of the indicated interacting (1, 2, 4, 8, 10, and 11) and noninteracting (3, 5, 6, 7, 9, and 12) TMPMs. The excitation and emission wavelengths of FITC were 480 and 510 nm, respectively. For confocal images of trapped NR, excitation and emission wavelengths of 510 and 580 nm were used. The experiment was conducted in RPMI 1640 medium in log phase (∼106) cells.