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. 2013 Apr 16;288(23):16775–16787. doi: 10.1074/jbc.M113.467159

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FIGURE 6. — A, binding of FITC-tagged TMPMs with the cell surface membrane of AD-CDR2 and AD-CaMDR1-overexpressing in S. cerevisiae cells as described under “Experimental Procedures.” B and C, TMPM8 inhibit NR accumulation in control (AD-CDR1) and AD-CDR1 + TMPM8 cells (B) and NR accumulation in AD-CDR2 and in AD-CDR2 + TMPM8 cells (C). The efflux of NR was carried out as described under “Experimental Procedures.” D, NR accumulation in AD-CaMDR1 and AD-CaMDR1 + TMPM8 cells. E, control cells (AD-CDR1) and AD-CDR1 + TMPM8. F, (AD-CDR2) and AD-CDR2 + TMPM8. G, AD-CaMDR1 and AD-CaMDR1 + TMPM8. The energy dependent NR efflux was initiated by the addition of 5 mm glucose (G). Values are means ± variations (error bars) for three independent experiments.