TABLE 3.

Effect of disulfide bond disruption on UCE activity

	Relative UCE activitya	Traffic to Golgib
WT	100	Yes
C51M	64.7 ± 5.2	Yes
C51M/C221L	9.7 ± 1.0	Yes
C115S	15.4 ± 1.6	Trace
C115S/C148V	ND	No
C132V	ND	No
C132V/C323L	ND	No

^a The values represent units of enzyme activity divided by the density of the band of mature UCE determined by Western blot analysis with WT set to 100. The values are the average of three determinations, each done in duplicate. ND not detected, indicating failure of protein to traffic to the Golgi and undergo furin cleavage.

^b “Yes” means transport to the Golgi is equivalent to WT as analyzed by Western blot.