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. 2013 Apr 22;288(23):16839–16847. doi: 10.1074/jbc.M113.459594

FIGURE 6.

FIGURE 6.

Hemoglobin-positive ratios of MEL cells with overexpression of Fut8 mutant. A, constructs of FUT8 mutant. RRM means R365A/R366A mutant of FUT8. FLM means flexible loop (D368A/K369A/V370A/G371A/T372A) mutant of FUT8. B, Western blot analysis of overexpression of FUT8 and FUT8 mutant. C, hemoglobin-positive ratio of MEL cells with the empty vector (control), overexpression of Fut8 and Fut8 mutants 4 days after 1.5% DMSO treatment. Individual circles indicate different cell clone. Four control cell clones, nine clones overexpressing Fut8, eight clones overexpressing RRM, and six clones overexpressing FLM were analyzed. The asterisk indicates statistically significant difference (p < 0.01).