Hemoglobin-positive ratios of K562 cells overexpressing FUT8 and FUT8 mutants.
A, Western blot analysis of FUT8 and FUT8 mutant overexpression. B, hemoglobin-positive ratios of K562 cells containing the empty vector (control), overexpressing FUT8, and overexpressing FUT8 mutant 4 days after 100 μm hemin treatment. Individual circles denote different cell clones. Three control cell clones, five clones overexpressing FUT8, and three clones overexpressing RRM were analyzed. The asterisk indicates statistically significant difference (p < 0.01).