TABLE 1.
Difference in gene expression between HD and LD MEL cells after drug treatment
Microarray results were filtered at an expression ratio of >1.5 or <0.66, p < 0.05, at more than one time point, and genes consistently up-regulated or down-regulated among the three chemical treatments were selected. The expression ratios are the average of quadruplicate experiments.
| Clone name | Gene symbol | Expression ratio (HD MEL cells/LD MEL cells) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DMSO |
HMBA |
TSA |
|||||||||||
| 6 h | 12 h | 24 h | 36 h | 6 h | 12 h | 24 h | 36 h | 6 h | 12 h | 24 h | 36 h | ||
| H3038C06 | Zfp951 | 0.48 | 0.38 | 0.39 | 0.24 | 0.42 | 0.53 | 0.42 | 0.25 | 0.62 | 0.64 | 0.48 | 0.45 |
| H3078E08 | unknown | 0.49 | 0.39 | 0.43 | 0.26 | 0.47 | 0.53 | 0.44 | 0.26 | 0.60 | 0.64 | 0.50 | 0.43 |
| H3049A07 | G430049J08Rik | 0.53 | 0.48 | 0.47 | 0.33 | 0.46 | 0.50 | 0.41 | 0.26 | 0.64 | 0.63 | 0.38 | 0.35 |
| H4070E06 | Unknown | 0.50 | 0.44 | 0.36 | 0.22 | 0.48 | 0.54 | 0.43 | 0.21 | 0.58 | 0.64 | 0.52 | 0.59 |
| H3062A11 | Clip2 | 0.59 | 0.46 | 0.51 | 0.33 | 0.45 | 0.51 | 0.47 | 0.29 | 0.76 | 0.69 | 0.46 | 0.41 |
| H4016D11 | Unknown | 0.59 | 0.55 | 0.46 | 0.34 | 0.55 | 0.61 | 0.49 | 0.27 | 0.60 | 0.59 | 0.46 | 0.49 |
| H3061H10 | Tmem123 | 0.66 | 0.62 | 0.73 | 0.48 | 0.47 | 0.61 | 0.45 | 0.27 | 0.67 | 0.61 | 0.44 | 0.36 |
| H3104H09 | Spc24 | 0.60 | 0.50 | 0.51 | 0.48 | 0.49 | 0.56 | 0.55 | 0.35 | 0.64 | 0.70 | 0.54 | 0.50 |
| H3073G11 | Rtp3 | 0.62 | 0.58 | 0.63 | 0.39 | 0.49 | 0.66 | 0.48 | 0.28 | 0.77 | 0.63 | 0.48 | 0.51 |
| H3078A01 | G430049J08Rik | 0.62 | 0.56 | 0.68 | 0.38 | 0.56 | 0.58 | 0.53 | 0.28 | 0.66 | 0.65 | 0.53 | 0.56 |
| H3046C10 | Idi1 | 0.68 | 0.56 | 0.95 | 0.61 | 0.50 | 0.57 | 0.80 | 0.61 | 0.50 | 0.49 | 0.53 | 0.56 |
| H4046D12 | Plek | 0.59 | 0.58 | 0.84 | 0.63 | 0.52 | 0.46 | 0.76 | 0.44 | 0.53 | 0.57 | 0.68 | 0.86 |
| H3037D02 | G430049J08Rik | 0.74 | 0.90 | 0.62 | 0.62 | 0.75 | 0.60 | 0.55 | 0.45 | 0.66 | 0.66 | 0.49 | 0.51 |
| H3078G11 | Drd3 | 0.76 | 0.85 | 0.94 | 0.58 | 0.63 | 0.66 | 0.59 | 0.32 | 0.75 | 0.68 | 0.49 | 0.54 |
| H4019B10 | Fut8 | 0.78 | 0.65 | 0.81 | 0.37 | 0.63 | 0.58 | 0.62 | 0.31 | 0.60 | 0.66 | 0.87 | 0.95 |
| H3078G10 | Rtp3 | 0.78 | 0.79 | 0.81 | 0.59 | 0.68 | 0.75 | 0.72 | 0.31 | 0.69 | 0.72 | 0.57 | 0.56 |
| H4073E05 | Rnpc2 | 0.72 | 0.77 | 0.89 | 0.66 | 0.46 | 0.71 | 0.85 | 0.57 | 0.72 | 0.93 | 0.55 | 0.48 |
| H3033F11 | Cst3 | 0.65 | 0.83 | 0.80 | 0.60 | 0.63 | 0.65 | 0.66 | 0.65 | 0.65 | 0.73 | 0.75 | 0.78 |
| H4040B02 | Gstt3 | 0.76 | 0.90 | 1.18 | 0.47 | 0.80 | 0.70 | 0.67 | 0.48 | 0.98 | 0.66 | 0.59 | 0.59 |
| H3138C09 | Rnf128 | 0.91 | 0.69 | 0.78 | 0.62 | 0.84 | 0.66 | 1.02 | 0.75 | 0.96 | 1.03 | 0.74 | 0.62 |
| H3032F06 | Hbb-y/Hbb-b1 | 0.61 | 0.69 | 0.79 | 0.92 | 0.57 | 0.68 | 1.14 | 1.54 | 0.62 | 0.60 | 0.69 | 0.96 |
| H4003H10 | Spn | 0.80 | 0.82 | 1.14 | 0.62 | 0.88 | 0.90 | 1.00 | 0.66 | 0.65 | 0.64 | 0.84 | 0.98 |
| H3113C01 | Hbb-y/Hbb-b1 | 0.69 | 0.59 | 0.87 | 1.07 | 0.68 | 0.75 | 1.39 | 1.59 | 0.60 | 0.68 | 0.86 | 0.96 |
| H3119H11 | Wdfy3 | 0.83 | 0.84 | 2.03 | 0.83 | 1.03 | 0.94 | 0.77 | 0.64 | 0.59 | 0.74 | 0.69 | 0.81 |
| H4020E10 | Il4ra | 0.95 | 0.89 | 0.90 | 0.60 | 1.06 | 1.00 | 1.00 | 0.54 | 0.88 | 1.17 | 1.33 | 1.66 |
| H3028F05 | Odc1 | 1.06 | 1.13 | 1.51 | 1.17 | 0.92 | 1.30 | 1.09 | 1.59 | 0.99 | 1.01 | 1.24 | 1.76 |
| H4062C02 | Mcm4 | 0.92 | 1.09 | 1.57 | 1.13 | 1.04 | 1.08 | 1.44 | 1.50 | 1.04 | 1.15 | 1.50 | 1.67 |
| H3024H12 | Hspa8 | 0.93 | 1.06 | 1.59 | 1.19 | 0.87 | 1.08 | 1.67 | 1.37 | 0.95 | 1.09 | 1.48 | 2.02 |
| H4006F09 | Hat1 | 1.12 | 0.94 | 1.68 | 1.27 | 1.08 | 1.12 | 1.69 | 1.38 | 1.01 | 1.55 | 1.30 | 1.61 |
| H3006C07 | Unknown | 1.23 | 1.24 | 1.50 | 1.16 | 1.24 | 1.65 | 1.37 | 1.57 | 0.96 | 1.06 | 1.39 | 1.80 |
| H4003E09 | Car2 | 1.05 | 1.15 | 1.63 | 1.34 | 1.01 | 1.08 | 1.82 | 1.65 | 0.87 | 0.90 | 1.79 | 2.19 |
| H3130A05 | Psmd2 | 1.26 | 1.20 | 1.67 | 1.44 | 1.19 | 1.17 | 1.85 | 1.50 | 1.27 | 1.11 | 1.48 | 2.10 |
| H3018A08 | Cct8 | 1.40 | 1.30 | 1.75 | 1.40 | 1.24 | 1.52 | 1.89 | 1.49 | 1.37 | 1.42 | 1.34 | 1.69 |
| H4034F12 | Fbxo45 | 1.33 | 1.28 | 2.14 | 1.21 | 1.22 | 1.43 | 1.65 | 1.30 | 1.30 | 1.63 | 1.39 | 2.09 |
| H3093D05 | Trfr | 1.26 | 1.42 | 1.77 | 1.51 | 1.30 | 1.28 | 1.72 | 2.06 | 1.23 | 1.17 | 1.62 | 1.68 |
| H3123F09 | Guf1 | 0.98 | 1.12 | 0.96 | 1.60 | 1.25 | 1.11 | 1.23 | 2.41 | 0.93 | 1.00 | 1.59 | 4.07 |
| H3122H09 | Hba-a1 | 0.97 | 1.19 | 1.10 | 1.69 | 1.21 | 1.07 | 1.37 | 2.85 | 1.13 | 1.01 | 1.44 | 3.50 |
| H3126A10 | Hba-a1 | 1.03 | 1.13 | 1.06 | 1.59 | 1.11 | 1.10 | 1.36 | 2.93 | 1.21 | 1.09 | 1.51 | 3.55 |
| H3026B04 | Ppat | 1.84 | 1.49 | 1.81 | 1.65 | 1.37 | 1.47 | 1.78 | 1.76 | 1.21 | 1.65 | 1.22 | 1.63 |
| H3126G09 | Hba-a1 | 1.00 | 1.08 | 1.05 | 2.13 | 1.18 | 0.95 | 1.35 | 3.39 | 1.05 | 1.06 | 1.34 | 3.65 |
| H3125H07 | Hba-a1 | 0.97 | 1.08 | 1.11 | 1.99 | 1.23 | 1.15 | 1.55 | 3.45 | 0.90 | 1.10 | 1.62 | 3.78 |
| H3121B01 | Hba-a1 | 1.04 | 1.23 | 1.31 | 2.05 | 1.43 | 1.11 | 1.63 | 3.81 | 1.06 | 1.08 | 1.68 | 3.97 |
| H3124F12 | unknown | 0.99 | 0.99 | 1.25 | 1.64 | 1.26 | 1.07 | 1.42 | 4.07 | 1.29 | 1.17 | 1.79 | 4.58 |
| H3122H11 | Hba-a1 | 1.02 | 1.20 | 1.29 | 2.03 | 1.25 | 1.30 | 1.40 | 4.25 | 1.17 | 1.03 | 1.85 | 4.27 |
| H3045A12 | Hba-a1 | 1.03 | 1.16 | 1.59 | 2.09 | 1.43 | 1.16 | 1.81 | 4.45 | 1.19 | 1.31 | 2.30 | 5.50 |
| H3123A01 | Hba-a1 | 1.06 | 1.40 | 1.90 | 2.73 | 1.25 | 1.32 | 2.27 | 5.18 | 1.35 | 1.01 | 1.64 | 4.09 |
| H3083F09 | Phtf1,Rsbn1 | 1.10 | 1.09 | 1.58 | 1.87 | 1.47 | 1.16 | 2.16 | 4.91 | 1.20 | 1.47 | 2.47 | 5.70 |
| H3123E05 | Hba-a1 | 1.34 | 1.30 | 1.90 | 2.72 | 1.51 | 1.49 | 2.33 | 5.70 | 1.42 | 1.22 | 2.02 | 4.85 |