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. 2013 Apr 23;288(23):16862–16871. doi: 10.1074/jbc.M113.464750

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — SPR analysis of the interaction of FPR-batroxobin and FPR-thrombin with immobilized γ_A/γ_A- or γ_A/γ′-fibrinogen. γ_A/γ_A- (●) and γ_A/γ′-fibrinogen (▴) were adsorbed on individual flow cells to ∼6000–8000 RU. Increasing concentrations (0–15 μm) of FPR-thrombin (A) or FPR-batroxobin (B) were successively injected into flow cells for 2 min, followed by a 4-min wash to monitor dissociation. The amount of protease bound at equilibrium (Req) after background correction is plotted against the input FPR-protease concentration. The insets show representative sensorgrams for the interaction of FPR-thrombin (A) and FPR-batroxobin (B) with γ_A/γ_A-fibrinogen in concentrations up to 15 and 6.4 μm, respectively. Data points represent the mean ± S.D. of 2- 3 experiments, and the lines represent nonlinear regression analyses.