Binding of 125I-YPR-batroxobin and 125I-YPR-thrombin to γA/γA- or γA/γ′-fibrin clots. 20 nm
125I-YPR-thrombin (A) or 40 nm
125I-YPR-batroxobin (B) was added to microcentrifuge tubes containing 0–8 μm γA/γA- (●) or γA/γ′-fibrinogen (▴), and 10 nm α-thrombin was used to initiate clotting. After incubation for 60 min, clots were pelleted by centrifugation, and free 125I-YPR-protease in the supernatant was used to calculate the bound fraction. Data are plotted as the percentage of 125I-YPR-protease bound to fibrin clots versus the fibrinogen concentration. Data points represent the mean ± S.D. of two experiments, each performed in duplicate, and the lines represent nonlinear regression analyses.