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. 2013 Apr 23;288(23):16862–16871. doi: 10.1074/jbc.M113.464750

FIGURE 3.

FIGURE 3.

Binding of 125I-YPR-batroxobin and 125I-YPR-thrombin to γAA- or γA/γ′-fibrin clots. 20 nm 125I-YPR-thrombin (A) or 40 nm 125I-YPR-batroxobin (B) was added to microcentrifuge tubes containing 0–8 μm γAA- (●) or γA/γ′-fibrinogen (▴), and 10 nm α-thrombin was used to initiate clotting. After incubation for 60 min, clots were pelleted by centrifugation, and free 125I-YPR-protease in the supernatant was used to calculate the bound fraction. Data are plotted as the percentage of 125I-YPR-protease bound to fibrin clots versus the fibrinogen concentration. Data points represent the mean ± S.D. of two experiments, each performed in duplicate, and the lines represent nonlinear regression analyses.