Dissociation constants for the binding of FPR-thrombin and FPR-batroxobin to fibrinogen, fibrin, and related fragments
The affinities of FPR-thrombin or FPR-batroxobin for immobilized fibrin(ogen), fibrinogen fragments, or peptides was determined using SPR. Kd values were determined by kinetic analysis of the binding sensorgrams. The interaction of thrombin with γA/γ′-fibrin(ogen) was analyzed using a two-site binding model. Each value represents the mean ± S.D. of two to three experiments. NB represents no binding.