TABLE 3.

Effect of Ca²⁺ on the membrane binding affinity of the C2 domain

Shown is a summary of kinetic constants for the binding of purified C2 domain to anionic lipids, in the presence of 150 mm NaCl, described in the legend to Fig. 5. Data represent weighted averages ± S.E.

Ca2+	kon (×1010)	koff	Kdcalc
μm	m−1s−1	s−1	nm
20	1.012 ± 0.009	3.88 ± 0.06	0.23 ± 0.02
200	2.241 ± 0.009	0.71 ± 0.02	0.031 ± 0.007