FIGURE 2.

Aβ oligomers impair the trafficking of BDNF-GFP endosomes. A, in the presence of Aβ, the average velocity of BDNF-GFP-containing endosomes was 1.73 ± 0.378 μm/s. This represented a 38.4 ± 13.4% (*, p < 0.01) decrease when compared with the average velocity of endosomes in the absence of velocities of BDNF-GFP-positive endosomes determined as described previously (22). B, distribution plot of the vesicle velocities reveal that in the presence of Aβ oligomers, the percentage of vesicle velocities >2 mm/s was greatly reduced (gray bars), and the majority of the vesicle velocity was <1 μm/s. C, representative time lapse image of BDNF-GFP containing endosomes demonstrates the amount of BDNF-TrkB complex that undergoes retrograde transport of the (from right to left). Scale bar, 10 μm.