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. 2013 Apr 24;288(23):16949–16959. doi: 10.1074/jbc.M112.420521

FIGURE 4.

FIGURE 4.

Mutational analyses of the residues identified for dsRNA binding and processing by the in vitro RNase assay and cell culture-based IFN suppression assay. A, WT and mutant LASV NP proteins were assessed for their exoribonuclease function in the in vitro RNase assay. A representative gel of at least three independent experiments was shown for each of the proteins. B, the ability of WT and mutant LASV NP proteins to suppress Sendai virus-induced IFN production was determined by a LUC-based IFN-β promoter assay. The results shown are the average of three independent experiments with error bars showing S.D. C, the levels of IFN-β and IFN-α produced from Sendai virus-infected A549 cells, in the presence of WT or mutant LASV NP proteins, were quantified by ELISA. The results shown are the average of three independent experiments with error bars showing S.D. Statistical analyses between the WT and mutant NP proteins were conducted using Student's t test with: ***, p < 0.001; **, p < 0.01.