Cellular localization of GFP-tagged Far3, Far7, Far8, or Far11 in the absence of individual components of the Far complex.
A, localization of Far3-GFP in the mutant strains as indicated (far3Δ, TPY1010; far3Δ far7Δ, TPY1358; far3Δ far8Δ, TPY1361; far3Δ far9Δ, TPY1348; far3Δ far10Δ, TPY1363; far3Δ far11Δ, TPY1402). B, localization of Far7-GFP in the mutant strains as indicated (far7Δ, TPY1013; far7Δ far3Δ, TPY1408; far7Δ far8Δ, TPY1366; far7Δ far9Δ, TPY1350; far7Δ far10Δ, TPY1368; far7Δ far11Δ, TPY1352). C, localization of Far8-GFP in the mutant strains as indicated (far8Δ, TPY1015; far8Δ far3Δ, TPY1369; far8Δ far7Δ, TPY1370; far8Δ far9Δ, TPY1351; far8Δ far10Δ, TPY1373; far8Δ far11Δ, TPY1405). D, localization of Far11-GFP in the mutant strains as indicated (far11Δ, SY4064; far11Δ far3Δ, TPY1374; far11Δ far7Δ, TPY1377; far11Δ far8Δ, TPY1406; far11Δ far9Δ, TPY1410; far11Δ far10Δ, TPY1379). All cells were grown in SD medium and observed by fluorescence microscopy.