FIGURE 4.

Uptake of cystatin C variants. Cells were incubated for 6 h with 1 μm recombinant human cystatin C. The total cystatin C content in lysates was measured by ELISA and correlated to the total protein content. Cystatin C content (ng/mg protein) in lysates of cells incubated in standard medium was subtracted. The resulting values were compared with the uptake of wild-type cystatin C in each experiment, which was set to 100%. Black symbols represent mean values of triplicate wells in a single experiment. Red bars represent median values (n = 3–6). For description of variants and the designation 219/214, see Fig. 1.