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. 2013 Apr 29;288(23):17019–17029. doi: 10.1074/jbc.M113.453449

FIGURE 6.

FIGURE 6.

Dose-dependent inhibition of intracellular enzyme activity after incubation with cystatin C. Cells were seeded in 96-well plates and incubated with standard medium or medium containing either 1 or 5 μm cystatin C for 6 h. After cell lysis a fluorescent substrate, specific for the different enzymes, was added. An end point value of the fluorescence was measured after 2.5 h. Bars represent mean values (n = 3–9) and are correlated to time and total protein content of the homogenate (fluorescence units/min/mg protein). The enzyme activity in lysates from cells incubated in medium without cystatin C addition was set to 100% in each experiment. As a control cells where incubated in standard medium and lysed before addition of cystatin C.