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Anti-GM-CSF autoantibody-containing plasma inhibits GM-CSF-induced MIP-1α protein expression. Normal PBMC incubated with normal or patient plasma and patient PBMC washed of autologous plasma were left unstimulated or stimulated with GM-CSF and fold-induction of MIP-1α production was determined. For each patient with anti-GM-CSF autoantibodies, values represent an average of 3 independent experiments.
