Fig. 2.
The cysteine biosynthesis gene serine-o-actetyl transferase (ScSAT) is constitutively higher expressed in leaves of the metallicolous isolated KH21 and thus displays a category (2) expression pattern. qPCR analysis of genotypes KH21 (full bars) and F20 (striped bars) after 2, 8 and 14 weeks in perlite cultures without (control, gray) and with 5 mg Zn L−1 and 0.5 mg Cd L−1 (Zn + Cd, black). Expression levels of ScSAT were normalized to ScACTIN2. Measurements were carried out in triplicates and values represent means ± SE of four biological replicates. Different letters represent significant differences (Student‘s t-test, p < 0.05).