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Zfhep ZHD binds the Oct-1 POU domain. 32P-ZHD (5 × 105 cpm) was incubated with 20 μl GST, GST-POUHD or GST-POU bound to glutathione-sepharose in the presence of 4 mg E. coli extract as competitor. Pellets were washed as described. 32P-ZHD bound by the fusion protein was eluted in SDS sample buffer, separated by 12.5 % SDS-PAGE and analyzed by autoradiography. Lane 1 received 5 % 32P-ZHD input in the reaction (Input). Full-length 32P-ZHD is indicated by the arrow.
