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. Author manuscript; available in PMC: 2013 Jun 7.
Published in final edited form as: Mol Biol Rep. 2003 Dec;30(4):199–206. doi: 10.1023/a:1026330907065

Fig 4.

Fig 4

ZHD interaction with Oct-1 POU domain is specific. 32P-ZHD (2.5 μl, approximately 2.3 × 105 cpm) was incubated with 7 μl glutathione-sepharose beads with GST-POU (or GST alone) with or without a 100-fold excess non-radioactive ZHD (250 μl). Beads were washed and eluted as described. The SDS-PAGE gel lanes received either 5 % 32P-ZHD input (Input), or 32P-ZHD eluted from the GST beads (GST), GST-POU beads (POU), or GST-POU beads with 100-fold excess non-radioactive ZHD (100x).