Fig. 6.

Activation of noncanonical NF-κB pathway by the stimulation of ds-RNA. A549 cells were treated with ds-RNA and TNF. The nuclear proteins were extracted and subjected to SID-SRM-MS analysis. A, the temporal profiles of nuclear p52 and p100 in the cells treated with dsRNA, derived from SID-SRM-MS analysis. B, the temporal profiles of nuclear p52 and p100 in the cells treated with TNF, derived from SID-SRM-MS analysis. C, the expression profiles of noncanonical NF-κB pathway-dependent NAF1 gene. Q-RT-PCR of NAF1 expression in the absence or presence of ds-RNA.