Table I. SRM parameters of SRM assays of proteins for sample amount normalization.
| Gene name | Swissprot number | Sequence | Q1 (m/z) | Q3 (m/z) | Ion type | CE (V) | Linear range | R2 | Lower limit of quantitation (amol) |
|---|---|---|---|---|---|---|---|---|---|
| IKKa | O15111 | IQLPIIQLR | 547.358 | 529.345 | y4 | 26 | 20,000 | 0.9996 | 50 |
| 547.358 | 642.429 | y5 | 23 | ||||||
| 547.358 | 739.482 | y6 | 19 | ||||||
| 547.358 | 852.566 | y7 | 18 | ||||||
| MAVS | Q7Z434 | VSASTVPTDGSSR | 632.312 | 719.331 | y7 | 20 | 20,000 | 0.9995 | 250 |
| 632.312 | 818.400 | y8 | 16 | ||||||
| 632.312 | 919.447 | y9 | 17 | ||||||
| 632.312 | 1006.479 | y10 | 20 | ||||||
| RelA | Q04206 | TPPYADPSLQAPVR | 756.396 | 867.504 | y8 | 27 | 20,000 | 0.9990 | 250 |
| 756.396 | 982.531 | y9 | 27 | ||||||
| 756.396 | 1053.568 | y10 | 27 | ||||||
| 756.396 | 1313.684 | y12 | 30 | ||||||
| IRF3 | Q14653 | LVGSEVGDR | 466.242 | 446.235 | y4 | 24 | 100,000 | 0.9998 | 50 |
| 466.242 | 575.278 | y5 | 26 | ||||||
| 466.242 | 719.331 | y7 | 26 | ||||||
| RIG-I | O95786 | VVFFANQIPVYEQQK | 905.480 | 891.457 | y7 | 26 | 2,000 | 0.9989 | 2500 |
| 905.480 | 1004.541 | y8 | 24 | ||||||
| 905.480 | 1132.599 | y9 | 26 | ||||||
| 905.480 | 1246.642 | y10 | 27 | ||||||
| TBK1 | Q9UHD2 | TTEENPIFVVSR | 696.362 | 817.493 | y7 | 24 | 20,000 | 0.9988 | 250 |
| 696.362 | 931.535 | y8 | 24 | ||||||
| 696.362 | 1060.578 | y9 | 23 | ||||||
| 696.362 | 1189.621 | y10 | 23 | ||||||
| IKBA | P259633 | LEPQEVPR | 484.264 | 628.341 | y5 | 20 | 20,000 | 0.9992 | 50 |
| 484.264 | 725.394 | y6 | 20 | ||||||
| 484.264 | 854.436 | y7 | 20 | ||||||
| 484.264 | 967.520 | y8 | 20 | ||||||
| IKKR | Q9Y6K9 | AQVTSLLGELQESQSR | 873.455 | 976.469 | y8 | 27 | 10,000 | 0.9984 | 500 |
| 873.455 | 1033.490 | y9 | 27 | ||||||
| 873.455 | 1146.574 | y10 | 27 | ||||||
| 873.455 | 1259.658 | y11 | 27 | ||||||
| 873.455 | 1346.690 | y12 | 27 | ||||||
| p52/p100 | Q00653 | DSGEEAAEPSAPSR | 701.808 | 743.368 | y7 | 20 | 20,000 | 0.9976 | 250 |
| 701.808 | 814.405 | y8 | 20 | ||||||
| 701.808 | 885.442 | y9 | 20 | ||||||
| 701.808 | 1014.484 | y10 | 27 | ||||||
| p100 | Q00653 | ALLDYGVTADAR | 632.83 | 689.357 | y7 | 20 | 20,000 | 0.9985 | 250 |
| 632.83 | 852.421 | y8 | 20 | ||||||
| 632.83 | 967.447 | y9 | 20 | ||||||
| 632.83 | 1080.531 | y10 | 21 |
Notes: Masses listed are for the native forms of the peptides.
CE, collision energy; Q, quadropole; R2, coefficient of determination.