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High accuracy MS and MS/MS information allows peptide identified with high confidence. (a) and (b) indicate the aligned MS/MS spectrum and mass error of fragmentation ions of a representative peptide RQHR↓GRGREPGAYPALDSRQE↓KR, respectively. The sequence of this fragment with classical dibasic cleavage sites was RQHR↓GRGREPGAYPALDSRQE↓KR, corresponding to the secretogranin-1 precursor.
