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Validation of the top over-expressed candidates using real-time PCR in LNCaP and LNCaP-SF cells. Seven of the 10 candidates (HMGCS2, DHRS2, AGR2, HSD11B2, ALDH6, MAOA, and MAOB) showed up-regulation in transcript levels in LNCaP-SF cells, and three (TYMP, ARG2, and SQSTM1) displayed no significant changes in mRNA expression. *p < 0.05, two-tailed t test.
