Table I. Antibodies and titers used for immunohistochemistry (IHC) and RPPA.
| Antigen | Source | Catalog no. | Type and dilution for IHC |
|---|---|---|---|
| VEGF | Dako, Glostrup, Denmark | M7273 | Murine mAb* 1:25 |
| CD31 | Dako, Glostrup, Denmark | M0823 | Murine mAb 1:80 |
| Proliferating cell nuclear antigen | Dako, Glostrup, Denmark | M7240 | Anti-Ki-67 mAb 1:50 |
| p85-cleaved PARP | Promega, Madison, WI | G7341 | Rabbit polyclonal 1:100 |
| Antigen | Source | Catalog no. | Type and dilution for RPPA |
|---|---|---|---|
| AKT | Cell Signaling | 2694 | Polyclonal Ab 1:500 |
| P473Ser-AKT | Cell Signaling | 9271 | Polyclonal Ab 1:500 |
| ERK | Cell Signaling | 9102 | Polyclonal Ab 1:500 |
| P202Thr/204Tyr-ERK | Cell Signaling | 4377 | mAb 1:1000 |
| EGF receptor | Cell Signaling | 4405 | mAb 1:200 |
| P845Tyr-EGF receptor | Cell Signaling | 2231 | Polyclonal Ab 1:200 |
| VEGFRII | Cell Signaling | 2479 | mAb 1:500 |
| PTyr1175-VEGFRII | Cell Signaling | 2478 | mAb 1:1000 |
| B-Raf | Cell Signaling | 9433 | mAb 1:1000 |
| P1177Ser-eNOS | Cell Signaling | 9570 | mAb 1:1000 |
| P38 | Abcam | ab7952 | Polyclonal Ab 1:1000 |
| Cleaved caspase 3 | Cell Signaling | 9661 | Polyclonal Ab 1:1000 |
| Cleaved PARP | Cell Signaling | 9541 | Polyclonal Ab 1:1000 |