Fig. 1.

Overview of quantitative phosphoproteome profiling. A, replication assay for WT and ΔssaR Salmonella within light and heavy labeled RAW264.7, respectively, 8 h after infection. Recovered bacteria were plated in a dilution series, and the number of CFU (colony-forming units) was determined. The mean (±S.D.) count of relative CFU was determined from three independent experiments. B, histograms of log₂-transformed SILAC ratios for all phosphosites quantified from RAW264.7 (black) and HeLa (gray). Dashed lines indicate the 95% confidence interval with no experimental perturbation. C, overlap of identified (top) and regulated (bottom) proteins between RAW264.7 and HeLa.