Table IV. Competitive and transient binders of desthiobiotin-ATP in normal cultures.
| Gene no. | Molecular mass | p value | Avg. NSAF_ATPγSa | Avg. NSAF_ATPb | Fold change | |
|---|---|---|---|---|---|---|
| Competitive Binders of Desthiobiotin-ATP | ||||||
| Acetyl-/propionyl-CoA carboxylase α subunit, AccA1 | Rv2501c | 71 kDa | (0.0000) | 1.00 | 15.67 | 15.67 |
| Glutamyl-tRNA amidotransferase subunit B, GatB | Rv3009c | 55 kDa | (0.0000) | 2.33 | 28.33 | 12.14 |
| Inosine-5-monophosphate dehydrogenase, GuaB3 | Rv3410c | 39 kDa | (0.0000) | 1.00 | 11.67 | 11.67 |
| Transmembrane serine/threonine-protein kinase H, PknH | Rv1266c | 67 kDa | (0.0000) | 1.00 | 11.33 | 11.33 |
| Fatty-acid-CoA ligase, FadD7 | Rv0119 | 55 kDa | (0.0000) | 1.00 | 9.67 | 9.67 |
| Phosphoribosylamine-glycine ligase, PurD | Rv0772 | 44 kDa | (0.0000) | 1.00 | 9.33 | 9.33 |
| Tryptophanyl-tRNA synthetase, TrpS | Rv3336c | 36 kDa | (0.0001) | 1.00 | 7.00 | 7.00 |
| Polyphosphate kinase, Ppk | Rv2984 | 83 kDa | (0.0000) | 3.33 | 22.67 | 6.80 |
| Phosphoglycerate kinase, Pgk | Rv1437 | 43 kDa | (0.0000) | 3.00 | 19.67 | 6.56 |
| Cysteinyl-tRNA synthetase 1, CysS1 | Rv3580c | 52 kDa | (0.0005) | 1.00 | 5.67 | 5.67 |
| Fatty-acid-CoA ligase, FadD23 | Rv3826 | 63 kDa | (0.0014) | 1.00 | 5.00 | 5.00 |
| Universal stress protein | Rv2319c | 32 kDa | (0.0012) | 1.33 | 6.33 | 4.75 |
| CTP synthase, PyrG | Rv1699 | 64 kDa | (0.0024) | 1.00 | 4.67 | 4.67 |
| Phosphofructokinase, PfkB | Rv2029c | 35 kDa | (0.0073) | 1.00 | 4.00 | 4.00 |
| ATP-dependent protease ATP-binding subunit, ClpC1 | Rv3596c | 94 kDa | (0.0000) | 15.33 | 61.00 | 3.98 |
| Anchored-membrane serine/threonine-protein kinase, PknF | Rv1746 | 51 kDa | (0.0130) | 1.00 | 3.67 | 3.67 |
| Chaperone protein, HtpG | Rv2299c | 73 kDa | (0.0130) | 1.00 | 3.67 | 3.67 |
| DNA polymerase I, PolA | Rv1629 | 98 kDa | (0.0078) | 1.67 | 6.00 | 3.60 |
| Acetate kinase, AckA | Rv0409 | 41 kDa | (0.0220) | 1.00 | 3.33 | 3.33 |
| UDP-N-acetylmuramoylalanyl-d-glutamyl-2,6-diaminopimelate-d-alanyl-d-alanyl ligase, MurF | Rv2157c | 52 kDa | (0.0220) | 1.00 | 3.33 | 3.33 |
| Conserved hypothetical protein | Rv3282 | 23 kDa | (0.0220) | 1.00 | 3.33 | 3.33 |
| Conserved hypothetical protein | Rv2510c | 57 kDa | (0.0220) | 1.00 | 3.33 | 3.33 |
| Fatty-acid-CoA ligase, FadD28 | Rv2941 | 63 kDa | (0.0077) | 2.33 | 7.67 | 3.29 |
| Nucleoside diphosphate kinase, NdkA | Rv2445c | 15 kDa | (0.0150) | 2.00 | 6.33 | 3.17 |
| 40-kDa secreted l-alanine dehydrogenase, Ald | Rv2780 | 39 kDa | (0.0066) | 3.00 | 9.33 | 3.11 |
| Adenosylhomocysteinase, SahH | Rv3248c | 54 kDa | (0.0017) | 5.00 | 15.00 | 3.00 |
| Pyruvate kinase, PykA | Rv1617 | 51 kDa | (0.0053) | 3.67 | 11.00 | 3.00 |
| Malate synthase G, GlcB | Rv1837c | 80 kDa | (0.0420) | 1.67 | 4.67 | 2.80 |
| Aldehyde dehydrogenase | Rv0458 | 55 kDa | (0.0350) | 2.67 | 7.00 | 2.63 |
| Adenylate kinase, Adk | Rv0733 | 20 kDa | (0.0035) | 17.33 | 36.67 | 2.12 |
| transmembrane serine/threonine-protein kinase D, PknD | Rv0931c | 70 kDa | (0.0400) | 8.67 | 17.67 | 2.04 |
| ATP synthase β chain, AtpD | Rv1310 | 53 kDa | (0.0250) | 13.33 | 26.33 | 1.98 |
| Transient binders of desthiobiotin-ATP | ||||||
| Endopeptidase ATP-binding protein chain B, ClpB | Rv0384c | 93 kDa | (0.0410) | 25.33 | 44.33 | 1.75 |
| 3-Hydroxyacyl-thioester dehydrogenase, HtdY | Rv3389c | 30 kDa | (0.4000) | 4.67 | 7.00 | 1.50 |
| Iron-regulated short-chain dehydrogenase/reductase | Rv3224 | 30 kDa | (0.4100) | 6.33 | 9.33 | 1.47 |
| Aminomethyltransferase, GcvT | Rv2211c | 40 kDa | (0.4300) | 4.33 | 6.33 | 1.46 |
| Phosphopantetheine adenylyltransferase, KdtB | Rv2965c | 18 kDa | (0.4700) | 2.33 | 3.33 | 1.43 |
| Chaperone protein, DnaK | Rv0350 | 67 kDa | (0.3800) | 40.00 | 57.00 | 1.43 |
| Cold shock protein A, CspA | Rv3648c | 7 kDa | (0.5300) | 5.67 | 7.67 | 1.35 |
| Secreted fibronectin-binding protein antigen | 85-B fbpB | Rv1886c | 35 kDa | (0.5400) | 2.00 | 2.67 |
| Iron-regulated peptidyl-prolyl cis-trans isomerase, PpiA | Rv0009 | 19 kDa | (0.5400) | 3.00 | 4.00 | 1.33 |
| Glutamine synthetase, GlnA2 | Rv2222c | 50 kDa | (0.5500) | 6.00 | 8.00 | 1.33 |
| Pyruvate dehydrogenase E2 component, SucB | Rv2215 | 57 kDa | (0.5500) | 7.33 | 9.67 | 1.32 |
| Acyl-CoA dehydrogenase, FadE5 | Rv0244c | 66 kDa | (0.5700) | 3.33 | 4.33 | 1.30 |
| Pyruvate dehydrogenase E1 component, AceE | Rv2241 | 100 kDa | (0.5700) | 4.67 | 6.00 | 1.29 |
| 60-kDa chaperonin 2, GroEL2 | Rv0440 | 57 kDa | (0.0180) | 279.00 | 352.00 | 1.26 |
| Iron-regulated conserved hypothetical protein | Rv1636 | 15 kDa | (0.3500) | 17.00 | 21.00 | 1.24 |
| Propionyl-CoA carboxylase β chain 5, AccD5 | Rv3280 | 59 kDa | (0.5800) | 3.00 | 3.67 | 1.22 |
| Low molecular weight protein antigen, Cfp2 | Rv2376c | 17 kDa | (0.5000) | 4.67 | 5.67 | 1.21 |
| Glutamine synthetase, GlnA1 | Rv2220 | 54 kDa | (0.3000) | 17.67 | 21.33 | 1.21 |
| Heat shock protein, HspX | Rv2031c | 16 kDa | (0.1800) | 36.00 | 43.33 | 1.20 |
| Succinyl-CoA synthetase β chain, SucC | Rv0951 | 41 kDa | (0.3500) | 11.67 | 14.00 | 1.20 |
| Oligoribonuclease, Orn | Rv2511 | 23 kDa | (0.4800) | 5.00 | 6.00 | 1.20 |
| Protein transport protei, SecE2 | Rv0379 | 8 kDa | (0.5400) | 3.33 | 4.00 | 1.20 |
| l-Lactate dehydrogenase, LldD2 | Rv1872c | 45 kDa | (0.4500) | 5.33 | 6.33 | 1.19 |
| Conserved hypothetical protein | Rv2159c | 36 kDa | (0.4300) | 5.67 | 6.67 | 1.18 |
| Bifunctional coenzyme A carboxylase α chain, AccA3 | Rv3285 | 64 kDa | (0.2800) | 13.67 | 16.00 | 1.17 |
| 50 S ribosomal protein L7/L12, RplL | Rv0652 | 13 kDa | (0.2500) | 16.00 | 18.67 | 1.17 |
| Conserved hypothetical protein | Rv3269 | 10 kDa | (0.4800) | 4.00 | 4.67 | 1.17 |
| Ribosome recycling factor, Frr | Rv2882c | 21 kDa | (0.5700) | 2.33 | 2.67 | 1.14 |
| Superoxide dismutase, SodA | Rv3846 | 23 kDa | (0.3900) | 5.33 | 6.00 | 1.13 |
| Transmembrane serine/threonine-protein kinase E, PknE | Rv1743 | 61 kDa | (0.3900) | 5.33 | 6.00 | 1.13 |
| Two-component system transcriptional regulator, DevR | Rv3133c | 23 kDa | (0.4600) | 3.33 | 3.67 | 1.10 |
| Tetrahydrodipicolinate N-succinyltransferase | Rv1201c | 33 kDa | (0.4600) | 3.33 | 3.67 | 1.10 |
| Leucyl-tRNA synthetase, LeuS | Rv0041 | 108 kDa | (0.3800) | 4.33 | 4.67 | 1.08 |
| Iron-regulated elongation factor tu tuf | Rv0685 | 44 kDa | (0.0300) | 40.33 | 43.33 | 1.07 |
| Immunogenic protein, Mpt64 | Rv1980c | 25 kDa | (0.3000) | 6.00 | 6.33 | 1.06 |
| 30 S ribosomal protein S1, RpsA | Rv1630 | 53 kDa | (0.2500) | 7.33 | 7.67 | 1.05 |
| Transmembrane serine/threonine-protein kinase A, PknA | Rv0015c | 46 kDa | (0.2100) | 8.67 | 9.00 | 1.04 |
| Transcriptional regulator | Rv0023 | 27 kDa | (0.5600) | 1.67 | 1.67 | 1.00 |
| Hypothetical protein, Wag31 | Rv2145c | 28 kDa | (0.4200) | 2.67 | 2.67 | 1.00 |
| Hypothetical protein | Rv3818 | 58 kDa | (0.5000) | 2.00 | 2.00 | 1.00 |
| Conserved alanine-rich protein | Rv2744c | 29 kDa | (0.2800) | 5.00 | 5.00 | 1.00 |
| Enoyl-CoA hydratase, EchA9 | Rv1071c | 36 kDa | (0.3900) | 3.00 | 3.00 | 1.00 |
| Conserved hypothetical protein | Rv2140c | 19 kDa | (0.5000) | 2.00 | 2.00 | 1.00 |
| Thiosulfate sulfurtransferase, CysA2 | Rv0815c | 31 kDa | (0.5600) | 1.67 | 1.67 | 1.00 |
| Zinc-type alcohol dehydrogenase NAD-dependent, AdhB | Rv0761c | 40 kDa | (0.2300) | 4.67 | 4.33 | 0.93 |
| Transcriptional regulator, MoxR1 | Rv1479 | 41 kDa | (0.2800) | 3.33 | 3.00 | 0.90 |
| 60-kDa chaperonin 1, GroEL1 | Rv3417c | 56 kDa | (0.0270) | 16.33 | 14.67 | 0.90 |
| Meromycolate extension acyl carrier protein, AcpM | Rv2244 | 13 kDa | (0.1400) | 6.33 | 5.67 | 0.89 |
| Haloalkane dehalogenase | Rv2296 | 33 kDa | (0.1500) | 6.00 | 5.33 | 0.89 |
| Succinyl-CoA synthetase α chain, SucD | Rv0952 | 31 kDa | (0.1600) | 5.67 | 5.00 | 0.88 |
| ATP synthase α chain, AtpA | Rv1308 | 59 kDa | (0.3300) | 2.33 | 2.00 | 0.86 |
| Integration host factor, MihF | Rv1388 | 21 kDa | (0.0001) | 41.33 | 34.67 | 0.84 |
| acyl-CoA dehydrogenase, FadE25 | Rv3274c | 42 kDa | (0.3500) | 2.00 | 1.67 | 0.83 |
| DNA polymerase III β chain, DnaN | Rv0002 | 42 kDa | (0.3500) | 2.00 | 1.67 | |
| Pyridoxamine 5-phosphate oxidase, PdxH | Rv2607 | 25 kDa | (0.1100) | 5.67 | 4.67 | 0.82 |
| 10-kDa chaperonin, GroES | Rv3418c | 11 kDa | (0.0000) | 50.33 | 40.67 | 0.81 |
| Iron-regulated aconitate hydratase, Acn | Rv1475c | 102 kDa | (0.1200) | 5.00 | 4.00 | 0.80 |
| 30 S ribosomal protein S16, RpsP | Rv2909c | 17 kDa | (0.3800) | 1.67 | 1.33 | 0.80 |
| Hypothetical protein, Cfp17 | Rv1827 | 17 kDa | (0.2000) | 3.33 | 2.67 | 0.80 |
| Fructose-bisphosphate aldolase, Fba | Rv0363c | 37 kDa | (0.2000) | 3.00 | 2.33 | 0.78 |
| Short-chain type dehydrogenase/reductase | Rv0148 | 30 kDa | (0.2000) | 3.00 | 2.33 | 0.78 |
| Transcriptional regulator, TetR family | Rv0144 | 31 kDa | (0.2000) | 3.00 | 2.33 | 0.78 |
| 19-kDa lipoprotein antigen precursor, LpqH | Rv3763 | 15 kDa | (0.0250) | 8.67 | 6.33 | 0.73 |
| Conserved hypothetical protein | Rv2406c | 15 kDa | (0.2100) | 2.33 | 1.67 | 0.71 |
| Electron transfer flavoprotein β subunit, FixA | Rv3029c | 28 kDa | (0.0039) | 13.67 | 9.67 | 0.71 |
| Enolase, Eno | Rv1023 | 45 kDa | (0.1300) | 3.33 | 2.33 | 0.70 |
a Average spectral count of desthiobiotin-labeled peptides was in the presence of 500 μm ATPγS. A value of 1 indicates 0 spectral counts in the presence of excess ATP analog.
b Average spectral count of desthiobiotin-labeled peptides with no excess ATPγS.