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. 2013 Mar 7;12(6):1661–1677. doi: 10.1074/mcp.M112.025288

Fig. 4.

Fig. 4.

miR-130a and miR-301a repress CSF-1 expression by binding a common target in CSF-1 mRNA 3[prime]UTR. A, miR-130a and miR-301a target the same region (2554–2576) in CSF-1 mRNA 3′UTR. B and C, miR-130a and miR-301a RNA levels are high in Bix3 cells compared with Hey and SKOV3 cells. D, secreted CSF-1 protein levels detected by ELISA. There is little secreted CSF-1 protein in Bix3 cells compared with Hey and SKOV3 cells. E, firefly luciferase (F-Luc) RNA ligated with either wild type full-length CSF-1 mRNA 3′UTR or one containing target point mutations of the common target were generated. F, SKOV3 ovarian cancer cells were transfected with the indicated F-Luc-CSF-1 3′UTR-WT or point mutant (F-Luc-CSF-1 3′UTR-Mut) constructs. Relative luciferase activity is the ratio of the activities of Firefly and the Renilla luciferases. n = 3. Mean ± S.D. is presented.