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Analysis of Lys27 and Lys36 methylation via quantitative mass spectrometry. Monomethylation of H3K27 is significantly down-regulated by ∼70% in ΔTXR1 cells (A), dimethylation of H3K27 is decreased by ∼40% (B), and trimethylation is decreased by ∼10% (C). Monomethylation of H3K36 might also be significantly affected, as suggested in D and E.
