Fig. 6.

Validation of PTM expression by means of Western blot. Rabbit polyclonal antibodies to H3K27Me1 (1:5000), H3K27Me2 (1:5000), and H3K27Me3 (1:5000) and mouse monoclonal antibody to H3K27Ac (1:50,000) were used to validate the PTM changes in wild-type and knockout cells with a 10-min exposure. The amount of H3 loaded was 0.5 μg. The elimination of TXR1 shows a significant reduction of H3K27Me1/Me2, whereas the knockout of ELZ2 leads to a reduction in H3K27Me2 and an undetectable signal in H3K27Me3. These results are consistent with the data from mass spectrometric quantification.