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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Nat Commun. 2013;4:1786. doi: 10.1038/ncomms2806

Fig. 1.

Fig. 1

Targeted deletion of Cx26 in DCs and OPCs in Cx26 cKO mice. a–c:Immunofluorescent labeling of the cochlear sensory epithelium for Cx26, Prestin, and Cx30 in the whole-mounting preparation. White-dashed lines represent OHC, DC and OPC area, where Cx26 labeling (green) is absent but Cx30 labeling (red color in panel c) remains in Cx26 cKO mice. d,e: Immunofluorescent staining of the cochlear cross-section for Cx26. OHCs are visualized by prestin labeling (red color). A white-dashed line box in panel e indicates OHCs, DCs, OPCs, and their neighboring area. OC: organ of Corti; LW: lateral wall; SLM: spiral limbus. f–h: High-magnification images of the boxed area. A white arrow in panel g indicates that the Cx26 labeling in the DC and OPC region is absent in Cx26 cKO mice. Three white arrows in panel h indicate 3 rows of OHCs. Scale bars = 50 μm in a–e; 10 μm in f–h.