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. Author manuscript; available in PMC: 2014 Apr 24.
Published in final edited form as: J Am Chem Soc. 2013 Apr 16;135(16):5946–5949. doi: 10.1021/ja400119t

Figure 4.

Figure 4

(A) Representation of the HER-2 affibodies (PDB code: 2B89) with chemically modified Cys positions [Cys42, Cys46] via stapling (10a) and alkylation (10); (B) CD spectra of 10 and 10a, and the corresponding temperature-dependent melting curves measured from the CD spectra at 222 nm (inset); (C) Biacore binding sensograms of 10, 10a with immobilized HER-2 extra-celluar binding region (adsorption/desorption time - 300 sec).