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. Author manuscript; available in PMC: 2014 May 24.
Published in final edited form as: Circ Res. 2013 Mar 18;112(11):1491–1505. doi: 10.1161/CIRCRESAHA.111.300436

Figure 4. Site-specific phosphorylation of protein kinase A (PKA)-target sites of cardiac myosin–binding protein-C (cMyBP-C).

Figure 4

A, Western blot analysis of cMyBP-C phosphorylation with specific antibodies for PKA sites Ser284 (top) and Ser275 (bottom) before (−) and after (+) PKA treatment (phosphorylation values were corrected for minor differences in protein loading by Ponceau-stained MLC2, myosin light chain 2). B, Protein phosphorylation values were normalized to the values found in untreated donor samples, which were included on the blot and set to 1 (dotted line). Thin-filament mutations were clustered in a single group (THINmut). Numbers of samples included in the analyses are indicated in the bar graphs. All samples showed an increased phosphorylation at both sites on PKA treatment.