Table 4.
Selected subcluster- or branch-identifying biomarkers observed by MALDI-TOF mass spectrometric analysis of V. vulnificus isolates from different MLST lineages
| Tentative protein identity | Group-specific peaks |
Peaks present in multiple groups |
|||||
|---|---|---|---|---|---|---|---|
| Exptl mass (avg m/z) | Predicted avg mass [(M + H)+], Da | Subcluster/branch specificity | Exptl mass (avg m/z) | Predicted avg mass [(M + H)+], Da | Presence in cluster/branch | Amino acid exchange | |
| 50S ribosomal protein L32 | 6,196 | 6,195.9 (Met loss) | BT 2 isolates | 6,168 | 6,167.9 (Met loss) | Most BT 1 isolates | K→R |
| Integration host factor subunit alpha | 10,705 | 10,705.2 (Met loss) | Cluster II isolates | 10,806 | NAa | Most cluster I isolates | NA |
| Putative sigma 54 modulation protein | 11,052 | 11,052.5 | Most cluster II isolates | 11,067 | 11,066.5 | Most cluster I isolates | T→S |
| Integration host factor subunit beta | 10,607 | 10,607.1 | Cluster IIA isolates | 10,592 | 10,593.0 | Cluster IIB and cluster I isolates | V→I |
| 30S ribosomal protein S17 | 9,477 | 9,477.1 (Met loss) | Cluster IIA isolates | 9,420 | NA | Cluster IIB and many cluster I isolates | NA |
| 50S ribosomal protein L24 | 11,100 | 11,100.9 (Met loss) | Cluster IIA isolates | 11,086 | NA | Cluster IIB and most cluster I isolates | NA |
| NA | 7,393 | NA | Cluster IIA isolates | 7,365 | NA | Cluster IIB and most cluster I isolates | NA |
| 50S ribosomal protein L31 | 8,070 | 8,070.2 | Cluster IIB isolates | 8,084 | 8,084.2 | Most cluster IIA and cluster I isolates | E→D |
a
NA, not assigned.